Ph.D. in Ingegneria Chimica , 32nd cycle (2016-2019)
Ph.D. obtained in 2020
Dissertation:
Freeze Drying of Therapeutic Proteins: A Simulation Approach to Optimize Formulation and Process Conditions (Abstract)
Tutors:
Roberto Pisano Antonello Barresi
Research presentation:
PosterProfile
Research topic
Freeze Drying of Therapeutic Proteins: A Simulation Approach to Optimize Formulation and Process
Research interests
Biography
development for biopharmaceuticals to be freeze dried. He also has an expertise in Molecular Dynamics (MD) simulations of biological systems, and physical chemistry in general. In 2018, he was a visiting researcher for six months at University of California, Santa Barbara, where he worked in the field of surface-driven denaturation of proteins and aggregation of amyloid-forming peptides. For this purpose, he used enhanced sampling techniques including replica echange MD (REMD), metadynamics and variationally enhanced sampling (VES). In the same year he also spent two months at the National Institute for Biological Standards and Control (Potters Bar, UK), where he investigated the effects of freezing and freeze drying on protein stability, with a particular focus on the role of controlled nucleation techniques. To achieve this result, he performed cell-based potency assays, size exclusion chromatography and dynamic light scattering on model systems, including the human growth hormone, interleukin-2, lactate dehydrogenase, myoglobin and factor VIII. Using a modelling approach, he also developed a design space tool for the freezing step of freeze drying, with the objective to optimize process conditions. During his Ph.D. he constantly aimed at bridging the gap between theoretical modeling and experiments in the field of protein stability, and biopreservation in general.
Awards and Honors
- premio miglior poster alla protein stability conference 2019 in Colorado (2019)
- Introductory Certificate in Project Management (2018)
- L'articolo "Application of the Quality by Design approach to the freezing step of freeze-drying: building the design space" è selezionato come 'featured article' dagli editori della rivista perchè ritenuto originale e significativo da un punto di vista scientifico. (2018)
- Legacy BioDesign LLC seleziona l'articolo 'Water Entrapment and Structure Ordering as Protection Mechanisms for Protein Structural Preservation' come particolarmente rilevante per gli esperti di formulazione e sviluppo di processo, e ne include una breve descrizione nella rivista 'Staying Current: Formulation of Biopharmaceuticals' (2018)
- Advances in Engineering seleziona l'articolo 'Clarifying the role of cryo- and lyo-protectants in the biopreservation of proteins' per il suo originale contenuto scientifico, e decide di dedicarvi un breve contributo. (2018)
Teaching
Teachings
Bachelor of Science
- Controllo, strumentazione e sicurezza per i processi chimici (modulo di Controllo e strumentazione per i processi chimici). A.A. 2018/19, INGEGNERIA CHIMICA E ALIMENTARE. Collaboratore del corso
Publications
Works published during the Ph.D. View all publications in Porto@Iris
- Arsiccio, A.; Giorsello, P.; Marenco, L.; Pisano, R. (2020)
Considerations on protein stability during freezing and its impact on the freeze-drying cycle: a design space approach. In: JOURNAL OF PHARMACEUTICAL SCIENCES, vol. 109, pp. 464-475. ISSN 0022-3549
Contributo su Rivista - Arsiccio, A.; Ganguly, P.; La Cortiglia, L.; Shea, J. -E.; Pisano, R. (2020)
ADD force field for sugars and polyols: predicting the additivity of protein-osmolyte interaction. In: JOURNAL OF PHYSICAL CHEMISTRY. B, CONDENSED MATTER, MATERIALS, SURFACES, INTERFACES & BIOPHYSICAL, vol. 124, pp. 7779-7790. ISSN 1520-6106
Contributo su Rivista - Arsiccio, A.; Marenco, L.; Pisano, R. (2020)
A model-based approach for the rational design of the freeze-thawing of a protein-based formulation. In: PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY, vol. 25, pp. 823-831. ISSN 1083-7450
Contributo su Rivista - Arsiccio, A.; Pisano, R. (2020)
The ice-water interface and protein stability: a review. In: JOURNAL OF PHARMACEUTICAL SCIENCES, vol. 109, pp. 2116-2130. ISSN 0022-3549
Contributo su Rivista - Arsiccio, A.; Matejtschuk, P.; Ezeajughi, E.; Riches-Duit, A.; Bullen, A.; Malik, K.; ... (2020)
Impact of controlled vacuum induced surface freezing on the freeze drying of human plasma. In: INTERNATIONAL JOURNAL OF PHARMACEUTICS, vol. 582. ISSN 0378-5173
Contributo su Rivista - Oddone, I.; Arsiccio, A.; Duru, C.; Malik, K.; Ferguson, J.; Pisano, R.; Matejtschuk, P. (2020)
Vacuum-induced surface freezing for the freeze-drying of the Human Growth Hormone: how does nucleation control affect protein stability?. In: JOURNAL OF PHARMACEUTICAL SCIENCES, vol. 109, pp. 254-263. ISSN 0022-3549
Contributo su Rivista - Arsiccio, Andrea (2020)
Freeze Drying of Therapeutic Proteins: A Simulation Approach to Optimize Formulation and Process Conditions. relatore: PISANO, ROBERTO; BARRESI, Antonello; , 32. XXXII Ciclo, P.: 286
Doctoral Thesis - Arsiccio, Andrea; Mccarty, James; Shea, Joan-Emma; Pisano, Roberto (2019)
Surface-driven denaturation of proteins during freeze-drying: An insight into the role of surfactants. In: 9th International Symposium on Lyophilization of Pharmaceuticals, Ghent, September 2-6, 2019, pp. 41-42
Contributo in Atti di Convegno (Proceeding) - Arsiccio, Andrea; Pisano, Roberto (2019)
A design space approach for the freezing step of freeze-drying: considerations for process performance and protein stability. In: 7th European Conference “EuroDrying’2019”, Torino, July 10-12, 2019
Contributo in Atti di Convegno (Proceeding) - Arsiccio, Andrea; Pisano, Roberto (2019)
Rational design of freeze-drying formulations: a molecular dynamics approach. In: 7th European Conference “EuroDrying’2019”, Torino, July 10-12, 2019
Contributo in Atti di Convegno (Proceeding) - Arsiccio, Andrea; Pisano, Roberto (2019)
Rational design of freeze-drying formulations: A Molecular Dynamics approach. In: 9th International Symposium on Lyophilization of Pharmaceuticals, Ghent, September 2-6, 2019, pp. 55-56
Contributo in Atti di Convegno (Proceeding) - Pisano, R.; Arsiccio, A.; Nakagawa, K.; Barresi, A. A. (2019)
Tuning, measurement and prediction of the impact of freezing on product morphology: A step toward improved design of freeze-drying cycles. In: DRYING TECHNOLOGY, vol. 37, pp. 579-599. ISSN 0737-3937
Contributo su Rivista - Arsiccio, Andrea; Mccarty, James; Shea, Joan-Emma; Pisano, Roberto (2019)
Effect of Surfactants on Surface-Induced Denaturation of Proteins: An Insight from Molecular Dynamics. In: 2019 Colorado Protein Stability Conference - 25th Anniversary, Breckenridge, Colorado, USA, July 29 - August 1
Contributo in Atti di Convegno (Proceeding) - Arsiccio, A.; Sparavigna, A. C.; Barresi, A. A. (2019)
Image segmentation applied to the investigation of craquelure domains in paintings. In: 7th European Drying Conference, Torino, 10-12 July 2019, pp. 547-554
Contributo in Atti di Convegno (Proceeding) - Arsiccio, A.; Sparavigna, A. C.; Pisano, R.; Barresi, A. A. (2019)
Measuring and predicting pore size distribution of freeze-dried solutions. In: DRYING TECHNOLOGY, vol. 37, pp. 435-447. ISSN 0737-3937
Contributo su Rivista